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The PIK3CA mutation is one of the most frequent mutations found in breast cancer and its positivity is correlated to a variety of clinical, prognostic and therapeutic implications. Within this proof-of-principle study, a novel method of detection is proposed using bespoke fluorogenic peptide nucleic acid (PNA) probes which promises a significant amount of benefits over classical diagnostic methods such as reduced costs, faster detection and increased ease of use. The methods adopted and difficulties encountered, as well as the limitations and other potential implementations of the procedure are discussed extensively.

The PIK3CA mutation is one of the most frequent mutations found in breast cancer and its positivity is correlated to a variety of clinical, prognostic and therapeutic implications. Within this proof-of-principle study, a novel method of detection is proposed using bespoke fluorogenic peptide nucleic acid (PNA) probes which promises a significant amount of benefits over classical diagnostic methods such as reduced costs, faster detection and increased ease of use. The methods adopted and difficulties encountered, as well as the limitations and other potential implementations of the procedure are discussed extensively.

Bespoke Peptide Nucleic Acid Optical Probes for the In-Situ Detection of Mutations in Breast Cancer Tumour Sections

MORRONI, ERMES
2020/2021

Abstract

The PIK3CA mutation is one of the most frequent mutations found in breast cancer and its positivity is correlated to a variety of clinical, prognostic and therapeutic implications. Within this proof-of-principle study, a novel method of detection is proposed using bespoke fluorogenic peptide nucleic acid (PNA) probes which promises a significant amount of benefits over classical diagnostic methods such as reduced costs, faster detection and increased ease of use. The methods adopted and difficulties encountered, as well as the limitations and other potential implementations of the procedure are discussed extensively.
DIPARTIMENTO DI MEDICINA MOLECOLARE
2020
Bespoke Peptide Nucleic Acid Optical Probes for the In-Situ Detection of Mutations in Breast Cancer Tumour Sections
The PIK3CA mutation is one of the most frequent mutations found in breast cancer and its positivity is correlated to a variety of clinical, prognostic and therapeutic implications. Within this proof-of-principle study, a novel method of detection is proposed using bespoke fluorogenic peptide nucleic acid (PNA) probes which promises a significant amount of benefits over classical diagnostic methods such as reduced costs, faster detection and increased ease of use. The methods adopted and difficulties encountered, as well as the limitations and other potential implementations of the procedure are discussed extensively.
LADAME, SYLVAIN
Lauree a ciclo unico
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/20.500.14239/13373