The aim of the present study is to evaluate and compare the cytotoxic effects of different bioceramic root canal sealers (FillRoot ST, BioRoot™RCS, Well-Root™ PT, CeraSeal, Pulp Canal Sealer™ EWT) on human gingival fibroblasts over a period of 24, 48 and 72 hours. Material and Methods: Immortalized human gingival fibroblast-1 (HGF-1) (1 × 10^4 cells) were incubated for 24 h at 37 °C with 100 μL of extraction medium made eluting each root canal sealer sample in cell culture medium for 24, 48 or 72 hours at 37 °C. Cells incubated with fresh medium was used as control. The percentage of viable cells in each well was calculated relative to control cells set to 100%. Results: All the tested bioceramic root canal sealers showed a mild cytotoxicity after 24 h, except for CeraSeal, which showed a moderate cytotoxicity after each time of incubation. The cell viability significantly reduced after incubation of 48 h and 72 h with FillRoot ST but did not significantly reduce after incubation of 48 h and 72 h with BioRoot™RCS and Well-Root™ PT. CeraSeal showed a moderate cytotoxicity after each time of incubation. The percentage of viable cells remained significantly lower if compared to BioRoot™RCS and Well-Root™ PT. Conclusions: FillRoot ST, BioRoot™RCS and Well-Root™ PT bioceramic sealers appears less cytotoxic at different periods after setting, compared with CeraSeal bioceramic sealers and Pulp Canal Sealer™ EWT traditional eugenol zinc-oxide sealer.
Lo scopo del presente studio è valutare e confrontare gli effetti citotossici di diversi sigillanti canalari bioceramici (FillRoot ST, BioRoot™RCS, Well-Root™ PT, CeraSeal, Pulp Canal Sealer™ EWT) sui fibroblasti gengivali umani per un periodo di 24, 48 e 72 ore. Materiali e metodi: I fibroblasti gengivali umani (HGF-1) (1 × 10^4 cellule) sono stati incubati per 24 ore a 37 ° C con 100 μL di mezzo di estrazione eluito con ogni campione di sigillante del canale radicolare nel mezzo di coltura cellulare per 24, 48 o 72 ore a 37 °C. Come controllo sono state utilizzate cellule incubate con terreno fresco. La percentuale di cellule vitali in ciascun pozzetto è stata calcolata rispetto alle cellule di controllo impostate al 100%. Risultati: tutti i sigillanti canalari bioceramici testati hanno mostrato una lieve citotossicità dopo 24 ore, ad eccezione di CeraSeal, che ha mostrato una moderata citotossicità dopo ogni tempo di incubazione. La vitalità cellulare si è ridotta significativamente dopo l'incubazione di 48 ore e 72 ore con FillRoot ST, ma non si è ridotta significativamente dopo l'incubazione di 48 ore e 72 ore con BioRoot™RCS e Well-Root™ PT. CeraSeal ha mostrato una moderata citotossicità dopo ogni tempo di incubazione. La percentuale di cellule vitali è rimasta significativamente inferiore rispetto a BioRoot™RCS e Well-Root™ PT. Conclusioni: i sigillanti bioceramici FillRoot ST, BioRoot™RCS e Well-Root™ PT appaiono meno citotossici in diversi periodi dopo l'indurimento, rispetto ai sigillanti bioceramici CeraSeal e al tradizionale sigillante all'ossido di zinco ed eugenolo Pulp Canal Sealer™ EWT.
Cementi endodontici bioceramici: analisi della citotossicità su fibroblasti gengivali umani
FRIJIO, ANDREA ANTONIO
2020/2021
Abstract
The aim of the present study is to evaluate and compare the cytotoxic effects of different bioceramic root canal sealers (FillRoot ST, BioRoot™RCS, Well-Root™ PT, CeraSeal, Pulp Canal Sealer™ EWT) on human gingival fibroblasts over a period of 24, 48 and 72 hours. Material and Methods: Immortalized human gingival fibroblast-1 (HGF-1) (1 × 10^4 cells) were incubated for 24 h at 37 °C with 100 μL of extraction medium made eluting each root canal sealer sample in cell culture medium for 24, 48 or 72 hours at 37 °C. Cells incubated with fresh medium was used as control. The percentage of viable cells in each well was calculated relative to control cells set to 100%. Results: All the tested bioceramic root canal sealers showed a mild cytotoxicity after 24 h, except for CeraSeal, which showed a moderate cytotoxicity after each time of incubation. The cell viability significantly reduced after incubation of 48 h and 72 h with FillRoot ST but did not significantly reduce after incubation of 48 h and 72 h with BioRoot™RCS and Well-Root™ PT. CeraSeal showed a moderate cytotoxicity after each time of incubation. The percentage of viable cells remained significantly lower if compared to BioRoot™RCS and Well-Root™ PT. Conclusions: FillRoot ST, BioRoot™RCS and Well-Root™ PT bioceramic sealers appears less cytotoxic at different periods after setting, compared with CeraSeal bioceramic sealers and Pulp Canal Sealer™ EWT traditional eugenol zinc-oxide sealer.È consentito all'utente scaricare e condividere i documenti disponibili a testo pieno in UNITESI UNIPV nel rispetto della licenza Creative Commons del tipo CC BY NC ND.
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https://hdl.handle.net/20.500.14239/13545