The aim of this work was to develop microstructured lipid carriers (MLCs) based on rice bran oil, a potent antioxidant, and containing Bis-Ethylhexyloxyphenol Methoxyphenyl Triazine, a broad-spectrum chemical sunscreen. The MLCs were prepared by the high-shear homogenization technique and were then characterized by microscopic and spectrophotometric analysis and encapsulation efficiency. The antioxidant properties of the microstructured carriers loaded with rice bran oil were assessed by means of a DPPH assay, an in vitro spectrophotometric assay. Finally, skin permeation was assessed by means of in vitro tests using vertical diffusion cells. The results obtained from this study showed that the use of rice bran oil, beeswax, candelilla wax and emulsifiers enabled the creation of regular-shaped MLCs with high encapsulation efficiency. In addition, these ingredients give the microparticles antioxidant properties and a pleasant tactile sensitivity. Analyses showed that the delivery of Bis-Ethylhexyloxyphenol Methoxyphenyl Triazine in MLCs resulted in increased protection against UV radiation due to the properties of the microstructured lipid carriers to reflect solar radiation, acting as a physical filter. In conclusion, this work represents a favorable technological strategy for the delivery of sunscreens, allowing an increase in protection against solar radiation with a reduction in the required UV filter concentration, with the aim of orienting the sun protection field towards a more sustainable approach.
Lo scopo di questo lavoro è stato quello di sviluppare vettori lipidici microstrutturati (MLC) a base di olio di crusca di riso, un potente antiossidante, e contenenti Bis-Ethylhexyloxyphenol Methoxyphenyl Triazine, un filtro solare chimico ad ampio spettro. Gli MLC sono stati allestiti con il metodo dell'omogeneizzazione ad alta forza di taglio e sono stati poi caratterizzati mediante analisi microscopica, spettrofotometrica ed efficienza di incapsulamento. Le proprietà antiossidanti dei carrier microstrutturati a base di olio di crusca di riso sono state valutate mediante un saggio DPPH, un saggio spettrofotometrico in vitro. Infine, la permeazione cutanea è stata valutata mediante test in vitro utilizzando celle a diffusione verticale. I risultati ottenuti da questo studio hanno dimostrato che l'uso di olio di crusca di riso, cera d'api, cera candelilla ed emulsionanti ha permesso di creare MLC di forma regolare con un'elevata efficienza di incapsulamento. Inoltre, questi ingredienti conferiscono alle microparticelle proprietà antiossidanti e una piacevole sensibilità tattile. Le analisi hanno dimostrato che la veicolazione di Bis-Ethylhexyloxyphenol Methoxyphenyl Triazine negli MLC può determinare una maggiore protezione contro le radiazioni UV, grazie alle proprietà dei veicoli lipidici microstrutturati di riflettere le radiazioni solari, agendo come un filtro fisico. In conclusione, questo lavoro rappresenta una strategia tecnologica favorevole per la veicolazione di filtri solari, consentendo un aumento della protezione contro le radiazioni solari con una riduzione della concentrazione di filtro UV richiesta, con l'obiettivo di orientare il campo della protezione solare verso un approccio più sostenibile.
Protezione solare e sostenibilità: sviluppo di microparticelle lipidiche a base di olio di crusca di riso
BIROLINI, FRANCESCA
2022/2023
Abstract
The aim of this work was to develop microstructured lipid carriers (MLCs) based on rice bran oil, a potent antioxidant, and containing Bis-Ethylhexyloxyphenol Methoxyphenyl Triazine, a broad-spectrum chemical sunscreen. The MLCs were prepared by the high-shear homogenization technique and were then characterized by microscopic and spectrophotometric analysis and encapsulation efficiency. The antioxidant properties of the microstructured carriers loaded with rice bran oil were assessed by means of a DPPH assay, an in vitro spectrophotometric assay. Finally, skin permeation was assessed by means of in vitro tests using vertical diffusion cells. The results obtained from this study showed that the use of rice bran oil, beeswax, candelilla wax and emulsifiers enabled the creation of regular-shaped MLCs with high encapsulation efficiency. In addition, these ingredients give the microparticles antioxidant properties and a pleasant tactile sensitivity. Analyses showed that the delivery of Bis-Ethylhexyloxyphenol Methoxyphenyl Triazine in MLCs resulted in increased protection against UV radiation due to the properties of the microstructured lipid carriers to reflect solar radiation, acting as a physical filter. In conclusion, this work represents a favorable technological strategy for the delivery of sunscreens, allowing an increase in protection against solar radiation with a reduction in the required UV filter concentration, with the aim of orienting the sun protection field towards a more sustainable approach.È consentito all'utente scaricare e condividere i documenti disponibili a testo pieno in UNITESI UNIPV nel rispetto della licenza Creative Commons del tipo CC BY NC ND.
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https://hdl.handle.net/20.500.14239/16024