Drug abuse is a chronic disease characterized by compulsive use of drugs and frequent relapses, associated to neuroadaptation of the reward circuit. Methamphetamine (METH) is a psycostimulat with an high addiction risk abused all over the world. Psycostimulat can affect neron morphology and dendritic spines in the hippocampus causing behavioral and motor alteration. Psychostimulants can also cause heavy metabolic alteration in neurons regarding glucose metabolism. Several studies showed that chronic consumpition of psychostimulants leads to important changes in the energetic cell metabolism. Acetyl-L-carnitin (ALC) is a substance that showed neuroprotective properties and the to as well be able to regulate glucose metabolism in cells. To further understand this mechanism we exposed primary cultures of different cell types to ALC and METH, and different parameters will be analysed with an important focus on how glucose transport and GLUT 3 expression are affected after treatment with ALC and METH both alone and combined. We will analyse cell viability, the effects on neuronal morphology (which has a direct impact on behaviour) as well.
La dipendenza da sostanze è una malattia cronica caratterizzata dall’uso compulsivo di droghe e da frequenti ricadute, che sono associate a neuroadattamenti nel reward circuit celebrale. La metanfetamina (METH) è uno psicostimolante ad alto rischio di dipendenza che viene abusato in tutto il mondo. Gli psicostimolanti influenzano la morfologia neuronale e le spine dendritiche nell’ippocampo causando alterazioni a livello motorio e comportamentale. Gli psicostimolanti causano anche ingenti alterazioni metaboliche nei neuroni soprattutto per quanto riguarda il metabolismo del glucosio. Diversi studi hanno dimostrato che l’assunzione cronica di psicostimolanti provochi cambiamenti importanti nel metabolismo energetico cellulare. L’ acetil-L-carnitina (ALC) è una sostanza che ha dimostrato di avere importati proprietà neuroprotettive e di essere capace di regolare il metabolismo glucidico delle cellule. Per meglio comprendere questi meccanismi abbiamo esposto diverse culture cellulari a ALC METH, e analizzato diversi parametri focalizzandoci principalmente su come il trasporto del glucosio e l’espressione di GLUT 3 sono influenzati dal trattamento con METH e ALC sia singolarmente che combinati. Saranno anche analizzati la mortalità cellulare e la morfologia neuronale.
L'effeto dell'Acetil-L-Carnitina su neuroni trattati con metamfetamina
VONELLA, MARIANNA
2022/2023
Abstract
Drug abuse is a chronic disease characterized by compulsive use of drugs and frequent relapses, associated to neuroadaptation of the reward circuit. Methamphetamine (METH) is a psycostimulat with an high addiction risk abused all over the world. Psycostimulat can affect neron morphology and dendritic spines in the hippocampus causing behavioral and motor alteration. Psychostimulants can also cause heavy metabolic alteration in neurons regarding glucose metabolism. Several studies showed that chronic consumpition of psychostimulants leads to important changes in the energetic cell metabolism. Acetyl-L-carnitin (ALC) is a substance that showed neuroprotective properties and the to as well be able to regulate glucose metabolism in cells. To further understand this mechanism we exposed primary cultures of different cell types to ALC and METH, and different parameters will be analysed with an important focus on how glucose transport and GLUT 3 expression are affected after treatment with ALC and METH both alone and combined. We will analyse cell viability, the effects on neuronal morphology (which has a direct impact on behaviour) as well.È consentito all'utente scaricare e condividere i documenti disponibili a testo pieno in UNITESI UNIPV nel rispetto della licenza Creative Commons del tipo CC BY NC ND.
Per maggiori informazioni e per verifiche sull'eventuale disponibilità del file scrivere a: unitesi@unipv.it.
https://hdl.handle.net/20.500.14239/16531