This study explores mosquito biology, focusing on Aedes albopictus, a disease-carrying mosquito species. The research uncovers the interaction between two critical proteins, LIPS-2 and Cp19, which play a pivotal role in mosquito biting behavior. The project involved the production, purification, and characterization of LIPS-2 and Cp19, revealing insights into their structural and functional aspects. Additionally, CRISPR-Cas9 technology was employed to create stable gene knockouts for these proteins, providing valuable insights into mosquito feeding behavior in vivo. This research offers a foundation for innovative vector control strategies and underscores the significance of targeting mosquito genes to disrupt disease transmission.
This study explores mosquito biology, focusing on Aedes albopictus, a disease-carrying mosquito species. The research uncovers the interaction between two critical proteins, LIPS-2 and Cp19, which play a pivotal role in mosquito biting behavior. The project involved the production, purification, and characterization of LIPS-2 and Cp19, revealing insights into their structural and functional aspects. Additionally, CRISPR-Cas9 technology was employed to create stable gene knockouts for these proteins, providing valuable insights into mosquito feeding behavior in vivo. This research offers a foundation for innovative vector control strategies and underscores the significance of targeting mosquito genes to disrupt disease transmission.
In vitro and in vivo exploration of LIPS-2 and Cp19 in Aedes albopictus: from protein characterization to CRISPR-Cas9 gene editing
d'ANGELO, GIORGIA
2022/2023
Abstract
This study explores mosquito biology, focusing on Aedes albopictus, a disease-carrying mosquito species. The research uncovers the interaction between two critical proteins, LIPS-2 and Cp19, which play a pivotal role in mosquito biting behavior. The project involved the production, purification, and characterization of LIPS-2 and Cp19, revealing insights into their structural and functional aspects. Additionally, CRISPR-Cas9 technology was employed to create stable gene knockouts for these proteins, providing valuable insights into mosquito feeding behavior in vivo. This research offers a foundation for innovative vector control strategies and underscores the significance of targeting mosquito genes to disrupt disease transmission.È consentito all'utente scaricare e condividere i documenti disponibili a testo pieno in UNITESI UNIPV nel rispetto della licenza Creative Commons del tipo CC BY NC ND.
Per maggiori informazioni e per verifiche sull'eventuale disponibilità del file scrivere a: unitesi@unipv.it.
https://hdl.handle.net/20.500.14239/16628