"Preparazione di Liquidi Ionici Supportati (SILs) e le loro applicazioni per l'isolazione delle immunoglobuline G"

ABSTRACT: After the first approval 1986 opens the market to the almost endless uses of monoclonal antibodies and Immunoglobulin-G uses. In fact, the advanced therapies market often has among its main players drugs that use Immunoglobulins-G. Since 1986, 44 IgG and monoclonal antibody products have been approved and placed on the pharmaceutical market in the advanced therapies market. However, their industrial-level production and purification, which includes high levels of purity, is still too expensive due to numerous limitations in the standardized conditions of purification techniques. Moreover, during affinity chromatography steps, the intent would be to avoid the use of canonical & standardized media such as protein A, which, despite being perfectly suitable, has several disadvantages. Therefore, it becomes clear how urgent the need is to find new IgG purification techniques. This justifies the choice of this study to observe and examine new affinity chromatography media for the purification of human immunoglobulin G, aiming to replace standardized techniques such as protein A in industrial Downstream processes for the purification of immunoglobulin G. In fact, as the title of the thesis announces, "Preparation of supported ionic liquids and their application for the isolation of immunoglobulin G" in this study, the preparation of liquid ionic supports for application in the isolation of immunoglobulins was observed. Therefore, the purpose of this dissertation is to research and analyse the relationship that can be achieved in the construction of SIL as an affinity chromatography support in the field of IgG immunoglobulin purification. The main objective of this work is to identify an adsorption interaction between supported ionic liquids (SILs), specifically the 2 different types: [Si][N3222] Cl and [Si][N3222OH] Cl. Specifically, the study focuses on analysing the differences between SIL[Si][N3222] Cl and the second [Si][N3222OH] Cl, as it is desired to evaluate the effect of the hydroxyl group present in the second SIL, which differentiates it from the first, which is assumed to intervene and play a role in the creation of weak interactions precisely with the IgG favouring its purification process.