While lung transplantation is the best option against many end-stage lung diseases, unfortunately in many situations the long- term outcome of this approach is negative. One of the most common cause of failure is chronic rejection due to the onset of bronchoalveolar obliterans syndrome (BOS) a pathology whose mechanism is still not completely understood. The disorder is characterized by a decrease in pulmonary functions (in particular of FEV1 value) and a rising airways obstruction, that leads patients to death. The main purpose of this project is to understand whether the presence of free elastases, in particular Human Neutrophil Elastase (HNE) in the bronchoalveolar lavage fluids (BALF) from BOS patients can be considered as a predictive factor for the development of the disease. This could mean that alpha1antitrypsin (AAT), the natural inhibitor of HNE and other elastases, for unknown reasons, cannot exert its inhibitory role of proteolytic enzymes which are present in excess during lung inflammation processes. We are currently working on the comparison of the proteomic profiles of BALF samples from BOS patients at different stages of severity to understand whether in these patients AAT and other anti-proteases still work as efficient inhibitors or not.
A PILOT MULTIFACTORIAL APPROACH TO INVESTIGATE α1-ANTITRYPSIN ACTIVITY AND ITS BALANCE WITH PROTEASES IN BRONCHOLAVEOLAR LAVAGE OF LUNG TRANSPLANT RECIPIENTS.
PELAMATTI, ERICA
2017/2018
Abstract
While lung transplantation is the best option against many end-stage lung diseases, unfortunately in many situations the long- term outcome of this approach is negative. One of the most common cause of failure is chronic rejection due to the onset of bronchoalveolar obliterans syndrome (BOS) a pathology whose mechanism is still not completely understood. The disorder is characterized by a decrease in pulmonary functions (in particular of FEV1 value) and a rising airways obstruction, that leads patients to death. The main purpose of this project is to understand whether the presence of free elastases, in particular Human Neutrophil Elastase (HNE) in the bronchoalveolar lavage fluids (BALF) from BOS patients can be considered as a predictive factor for the development of the disease. This could mean that alpha1antitrypsin (AAT), the natural inhibitor of HNE and other elastases, for unknown reasons, cannot exert its inhibitory role of proteolytic enzymes which are present in excess during lung inflammation processes. We are currently working on the comparison of the proteomic profiles of BALF samples from BOS patients at different stages of severity to understand whether in these patients AAT and other anti-proteases still work as efficient inhibitors or not.È consentito all'utente scaricare e condividere i documenti disponibili a testo pieno in UNITESI UNIPV nel rispetto della licenza Creative Commons del tipo CC BY NC ND.
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https://hdl.handle.net/20.500.14239/20469