EXPERIMENTAL STUDY ON THE USE OF TOBRAMYCIN LOADED LYOPHILIZED LIPOSOMES: CHARACTERIZATION AND EFFICACY EVALUATION.

This thesis investigates the development and evaluation of a lyophilized liposomal drug delivery system encapsulating tobramycin, aimed at enhancing the antibiotic's stability and efficacy. Liposomes were produced using microfluidic technology and characterized for size, charge, morphology, and encapsulation efficiency. A Design of Experiments (DoE) statistical approach was employed to optimize formulation parameters such as cholesterol content, PEGylation, and trehalose concentration. The liposomes were freeze-dried and rehydrated to assess their structural integrity and antimicrobial performance. Results demonstrated that optimized formulations retained desirable physicochemical properties and exhibited effective antibacterial activity against E. coli, confirming the potential of lyophilized liposomes as a promising delivery platform for tobramycin.