PROGETTAZIONE DELL'APPARECCHIATURA MECCANICO DI UN BIOREATTORE

Bioreactors are an important inevitable part of any tissue engineering (TE) strategy as they aid the construction of three-dimensional functional tissues. Since the aim of a bioreactor is to create a biological product, the engineering parameters, for example, internal and external mass transfer, fluid velocity, shear stress, electrical current distribution, and so forth, are worth to be thoroughly investigated. The effects of such engineering parameters on biological cultures have been addressed in only a few preceding studies. Furthermore, it would be highly inefficient to determine the optimal engineering parameters by trial-and-error method. A solution is provided by emerging modeling and computational tools and by analyzing oxygen, carbon dioxide, and nutrient and metabolism waste material transports, which can simulate and predict the experimental results. Discovering the optimal engineering parameters is crucial not only to reduce the cost and time of experiments, but also to enhance efficacy and functionality of the tissue construct. This review intends to provide an inclusive package of the engineering parameters together with their calculation procedure in addition to the modeling techniques in TE bioreactors. A bioreactor provides a controllable environment enabling the biological, biochemical, and biomechanical requirements to manufacture engineered products. As the bioreactor aims to create a desired biological product. Understanding of the mass transfer behaviors in bioreactors would result in improved reactor designs, reactor operation, and modelling tools, which are important for maximizing reaction rates, optimizing throughput rates, and minimizing cost. The paper discusses the bioreactor design and various types of bioreactors, which are useful for industrial operations. A basic understanding of mass transfer and its underlying principles is essential to single-use bioreactor design, determining bioreactor operating parameters, and optimizing culture conditions. In this thesis, we have discussed the design of the electrical equipment of a bioreactor in detail the construction and working of the electrical equipment which is used in the construction of a bioreactor. A bioreactor provides a controllable environment enabling the biological, biochemical, and biomechanical requirements to manufacture engineered products. As the bioreactor aims to create a desired biological product, it is important to closely monitor the reaction parameters like internal and external mass transfer, heat transfer, Fluid velocity, shear stress etc. The effects of such reaction variables on biological cultures and analyzing the other parameters such as oxygen, carbon dioxide, nutrient and metabolism waste material transports have been addressed in the paper. Sophisticated and sound bioreactor design with unique performance characteristics is essential in production of useful biotechnological products from natural and genetically modified cell systems. Understanding of the mass transfer behaviors in bioreactors would result in improved reactor designs, reactor operation, and modelling tools.